山东大学生命科学学院导师介绍：祁庆生

　　祁庆生 教授
　　博士生导师
　　Tel: +86-531-88365628
　　E-mail:qiqingsheng@sdu.edu.cn
　　
　　教育背景
　　Prof. Dr. Qingsheng Qi
　　State Key Lab of Microbial Technology
　　Life Science School, Shandong University
　　Shanda Nanlu 27
　　250100, Jinan PR.China
　　德国明斯特大学微生物系博士，2001年被聘为德国开姆尼茨工业大学生物工程系课题组负责人。2004年回国任山东大学微生物技术国家重点实验室教授，博士生导师；山东大学国家糖工程中心研究员。教育部新世纪优秀人才。《生物工程学报》编辑，生物化学学会复合糖专业委员会委员。现在主持国家自然科学基金，教育部重点项目，国家863项目, 973子项目等多项。在包括《Applied Environmental Microbiology》,《Journal of Biological Chemistry》,《FEMS Microbiology Letters》 在内的国际著名学术期刊上发表SCI论文40余篇，总被引用次数250多次。获得专利2项，申请4项。
　　讲授酶学，糖生物学和代谢工程课程，2009年拟招收微生物学和发酵工程博士2人。
　　
　　研究方向
　　1.通过代谢途径的建立和改造实现生物基化学品及生物可降解聚合物的合成。通过改造微生物的代谢，在重组大肠杆菌中生产重要工业产品，主要包括聚羟基脂肪酸、琥珀酸等有机酸。
　　2.通过改造微生物表达系统来生产糖基化改造的人源化糖蛋白和抗体以及稀有糖类；利用代谢工程合成小分子糖药物。药物的环糊精，脂质体包裹及运输。
　　3.建立微生物生物合成代谢的调控方法。通过全调控实现微生物（主要是大肠杆菌和酵母）抗性的提高及表达代谢能力的提高。研究微生物代谢的机理。
　　
　　研究成果及发表论文
　　1.Kang, Z., Wang, Q., Zhang, H., Qi, Q* (2008) Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production, Appl. Microbiol. Biotechnol. DOI 10.1007/s00253-008-1428-z
　　2.Xin, F., Wang, S., Song, L., Liang, Q., Qi, Q* (2008) Molecular identification and characterization of peptide: N-glycanase from Schizosaccharomyces pombe. Biochem Biophys Res Commun, online (doi:10.1016/j.bbrc.2008.02.017)
　　3.Wang, Q., Li, L., Chen, M., Qi, Q. Wang, PG. (2008) Construction of a Novel Pichia pastoris Cell-Surface Display System Based on the Cell Wall Protein Pir1.Curr Microbiol. 56:352-357.
　　4.信封学，王鹏，祁庆生 （2008）粟酒裂殖酵母N-糖酰胺酶在大肠杆菌中的表达、纯化及活性分析，24（4）。
　　5.Qingjie Wang#, Lei Li#, Min Chen, Peng George Wang*, Qingsheng Qi (2007) Construction of a novel system for cell surface display of heterologous protein on Pichia pastoris Biotechnology Lett. 29:1561-1566.
　　6.Dawei Zhang, Peng George Wang*, Qingsheng Qi* (2007) A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli Biotechnology lett.29:1729-1733.
　　7.Jungang Zhou, Houcheng Zhang, Xianwei Liu, Min Chen, Peng George Wang*, Qingsheng Qi* Deficiency in N-glycan outer chain elongation accelerates cell death in Saccharomyces cerevisiae. Current Microbiology. 55:198-204.
　　8.Li, R., Chen, Q., Wang, P., Qi, Q*. (2007) A novel-designed Escherichia coli for the production of various polyhydroxyalkanoates from inexpensive substrate mixture. Appl. Microbiol Biotechnol. 75:1103-1109.
　　9.Qi,Q. Mokhtar MN., Zimmermann, W. (2007) Effect of ethanol on the synthesis of large-ring cyclodextrins by cyclodextrin glucanotransferases J Incl Phenom Macrocycl Chem 57:95–99
　　10.Su, Y., Zhang, H., Gu, L., Chen, M., Xiao, M., Wang, PG., Qi, Q.* (2007) Construction of engineered yeast for the hydrolysis of glycoprotein with N-glycosidase on the cell surface. Enzyme and Microbiol Technology, 40: 1496-1502.
　　11.Wang S, Wang PG, Qi Q.* (2007) Influence of Substrate Conformation on the Deglycosylation of Ribonuclease B by Recombinant Yeast Peptide:N-glycanase. Acta Biochim Biophys Sin (Shanghai).39(1):8-14.
　　12.Li, R., Zhang, H., Qi, Q.* (2007) Production of polyhydroxyalkanoates in recombinant Escherichia coli. Bioresource Technol. 98: 2313-2320.
　　13.Wang, Z., Qi, Q.,* Wang, P.(2006) Engineering of Cyclodextrin Glucanotransferase on the Cell Surface of Yeast for improved cyclodextrin production. Appl. Environ. Microbiol. 72: 1873-1877.
　　14.Zhang, H., Wang, P. Qi, Q.* (2006) Molecular effect of FadD on the regulation and metabolism of fatty acid in Escherichia coli. FEMS Microbioloy Lett. 259:249-253.
　　15.Chen C, Shi Y, Li S, Qi Q, Gu L, Song J, Wang PG. (2006) A glycosylated nitric oxide donor, beta-Gal-NONOate, and its site-specific antitumor activity. Arch Pharm (Weinheim). 339(7):366-71.
　　16.Chen, C., Shi, Y., Song, J., Qi, Q., Gu L., Wang, PG. (2006) Delivery of Nitric Oxide Released from β-Gal-NONOate Activation by β-Galactosidase and Its Activity against Escherichia coli Biological & Pharmaceutical Bulletin, 26, 1239-1241.
　　17.Zhang, H., Bi, J., Chen, C. Huang, G., Qi, Q., Xiao, M. and Wang, PG (2006) Immobilization of UDP- galactose 4- epimerase from Escherichia coli on the Yeast Cell Surface. Biosci. Biotechnol. Biochem. 70:2303-6.
　　18.Qi,Q.*, Zimmerman, W (2005) Cyclodextrin glucanotransferase: from gene to application. Appl. Microbiol. Biotechnol.66:475-485.
　　19.Chen, M., Liu, X., Wang, Z., Song, J., Qi, Q.*, Wang, PG (2005) Modification of Plant N-glycans Processing: The Future of Producing Therapeutic Protein by Transgenic Plants. Medicinal research reviews. 25:343-360.
　　20.Song, J. Wang, PG. Cai, T. Liu, Z. Gu, L. Qi, Q. (2004) Novel sialic acid/NO conjugate as potential anti-influenza drugs. NITRIC OXIDE-BIOLOGY AND CHEMISTRY. 11: 56-56.
　　21.Qi, Q., She, X. Endo, T. Zimmermann, W. (2004) Effect of the reaction temperature on the cyclization and coupling reaction of the cyclodextrin glucanotransferase from Bacillus macerans for the synthesis of large ring cyclodextrin. Tetrahedron. 60:799-806.
　　22.Rehm, B. H. A., Hoffmann, N., Qi, Q., Fiedler, S., Steinbüchel, A. (2004) Biosynthesis of latex-like polyhydroxyalkanoates, in Proceedings of the International Symposium on Bioconversion of renewable raw materials, GBF, Braunschweig, Germany. Pp.163-175.
　　23.Qi, Q. Zimmermann, W. (2003) Preparation of a cyclodextrin glucanotransferase from bacterial isolate BT3-2: towards the production of large ring cyclodextrin. Chem. Listy: 97:362.
　　24.Hoffmann, N., Amara, A., Beermann, B., Qi, Q., Hinz HJ., Rehm BH. (2002) Biochemical characterization of the Pseudomonas putida 3-hydroxyacyl-ACP:CoA transacylase, which diverts intermediates of fatty acid de novo biosynthesis. J Biol Chem. 277: 42926-42936.
　　25.Rehm, BHA., Qi, Q., Beermann, BB., Hinz, HJ., Steinbüchel, A. (2001) Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli. Biochem J. 358:263-8.
　　26.Qi, Q., Rehm, B. H. A. (2001) Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: Molecular characterization of the polyhydroxybutyrate synthase and evidence for intracellular mobilization. Microbioloy.147：3353-3358.
　　27.Qi, Q., Steinbüchel, A., Rehm, B. H. A. (2000) In vitro synthesis of poly(3-hydroxydecanoate): purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa, Appl. Microbiol. Biotechnol. 54:37-43.
　　28.Steinbüchel, A., Brämer, C., Füchenbusch, B., Gorenflo, V., Hein, S., Jossek, R., Kalscheuer, R., Langbach, S., Qi, Q. (1998) Poly(hydroxyalkanoaic acid) Biosynthesis Pathways. 35-47. In A. Steinbüchel (ed.), Biochemical principles and mechanisms of biosynthesis and biodegradation of polymers. Whiley-VCH, New York, N.Y.
　　29.Qi, Q., Steinbüchel, A., Rehm, B. H. A. (1998) Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): inhibition of fatty acid -oxidation by acrylic acid. FEMS Microbiol. Lett. 167:89-94
　　30.Qi, Q., Rehm, B. H. A., Steinbüchel, A. (1997) Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2. FEMS Microbiol. Lett. 157:155-162
　　31.周峻岗 张厚程 王鹏 祁庆生（2007） Man8GlcNAc2糖基化的酿酒酵母菌株的构建，微生物学报，接受。
　　32.张大伟，王鹏，祁庆生（2007）利用重组大肠杆菌进行寡糖合成的研究进展，生物工程学报，23:21-25
　　33.段青，王倩，祁庆生* （2007）绿色荧光蛋白在蛋白质研究中的应用，生命的化学，27:48-50
　　34.赵新帅，王占坤，祁庆生*（2007）环糊精糖基转移酶产物专一性改造：难题与挑战，生物工程学报，23:181-188
　　35.苏移山, 王圣钧, 王鹏, 祁庆生* （2005）N-糖酰胺酶F在大肠杆菌中的高效表达及其脱糖基化作用, 生物工程学报, 6:911-915。
　　36.尹瑶, 王圣钧, 苏移山, 祁庆生* （2005）真核生物糖蛋白合成的质量控制——糖酰胺酶的作用, 生命的化学, 5:360-362。
　　37.庄国强，李爱英，文欣，祁庆生，许平，曲音波(1999) 利用DO-stat补料分批培养的方法累聚β-羟基丁酸的研究. 环境科学学报 19(1). 6-10.
　　38.许平，马翠卿，祁庆生，沈亚领 (1999) 爱德华氏菌产乳酸氧化酶的条件研究。微生物学报 13，234-236
　　39.祁庆生，刘复今，肖敏(1996) 己酸菌LII的生理及代谢特性。微生物学通报12, 1-5。

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