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南京农业大学园艺学院导师介绍:柳李旺

 


姓名:

柳李旺

性别:

 

出生日期:

1972.10

职称:

教授

学历:

研究生

学位:

博士

毕业院校:

南京农业大学

学科专业:

蔬菜学

任课名称:

园艺作物育种学、园艺科研技能训练、植物组织培养、现代蔬菜科学研究技术(研究生)

电话:

+86-25-84395563

Email:

nauliulw@yahoo.com.cn

发表论文:

发表论文
Xu Liang, Wang Liangju, Gong Yiqin , Dai Wenhao, Wang Yan, Zhu Xianwen, Wen Tiancai, Liu Liwang *. Genetic linkage map construction and QTL mapping of cadmium accumulation in radish. Theor Appl Genet, 2012,4, DOI 10.1007/s00122-012-1858-y(SCI) (通讯作者)
Sun Xinju, Liu Yang, Wang Liangju, Xianwen Zhu, Gong Yiqin, Liu Liwang *. Molecular characterization of the Rs-Rf1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration. Mol Breeding,2012,6. DOI 10.1007/s11032-012-9756-1(SCI)(通讯作者)
Lina Jiang, Liangju Wang, Liwang Liu*, Xianwen Zhu, Lulu Zhai, Yiqin Gong*. Development and characterization of cDNA library based novel EST-SSR marker in radish (Raphanus sativus L.). Scientia Horticulturae, 2012,140 :164–172(SCI) (通讯作者)
Zhang Yanyu,Xu Liang,Zhu Xianwen,Yiqin Gong,Xiang Fei,Sun Xiaochuan,Liu Liwang*.Proteomic analysis of heat stress response in leaves of radish (Raphanus sativus L) .Plant MolecularBiology Reporter ,2012,DOI 10.1007/s11105-012-0486-7(SCI)(通讯作者)
Xu Yuanyuan, Zhu Xianwen, Gong Yiqin, Xu Liang, Wang Yan , Liu Liwang*.Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR.Biochemical and Biophysical Research Communication (BBRC),2012,DOI 10.1016/j.bbrc.2012.06.119 (SCI)(通讯作者)
Wang, K., Sha, Y., Gong, Y., Xu, L., Liu, L*., Zhu, X. and Huang, D. 2012. Karyotype analysis and molecular markers identification of radish (Raphanus sativus L.) germplasm. Acta Horticulturae (ISHS) 929:25-221 (ISTP) (通讯作者)
Yan Wang, Xiaoyan Li, Jian Xu, Yuanyuan Xu, Liwang Liu . 2011. cultivar identification and genetic diversity analysis of cauliflower with molecular markers . Acta Hort. (ISHS) 918:315-321
LIU Li-Wang*, ZHAO Li-Ping, GONG Yi-Qin, WANG Ming-Xia, CHEN Li-Ming, YU Fan-Min, WANG Long-Zhi. et al., DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers, Scientia Horticulturae ,2008,116 :240–247(SCI)(通讯作者).
Liu.LW, GuoW, Zhu X, Zhang T. Inheritance and Fine Mapping of Fertility Restoring Gene for Cytoplasmic Male Sterility in Gossypium. Theor Appl Genet, 2003,106:461-469(SCI)
Liu L. Genotypic Representation Analysis (GRA):A new strategy for identifying molecular marker tightly linked to important gene. J Genetics and Molecular Biology.2004,15(3/4): 205-209(通讯作者)
L. Liu*, Zhu, X., Gong, Y., Song, X., Wang, Y., Zhao, L.,Wang, L. Gentic diversity analysis of radish germplasm with RAPD, AFLP and SRAP markers. Acta Horticulturae (ISTP) 2007, 760:125-130 (通讯作者)
Li-Wang Liu *, Yan Wang, Yi-Qin GONG , Tong-Min Zhao, Xiao-Yan Li, Fan-Min Yu. Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers. Scientia Horticulturae,2007 ,115:7–12 (SCI) (通讯作者)
Liwang Liu*, Guang Liu, Yiqin Gong, Wenhao Dai, Yan Wang, Fanmin Yu, and Yunying Ren. Evaluation of Genetic Purity of F1 Hybrid Seeds in Cabbage with RAPD, ISSR, SRAP, and SSR Markers. Hortsci., 2007,42(3) : 724-727 (SCI) (通讯作者)
Yiqin Gong, Wei Wang,Liwang Liu*,Yan Wang, Zange Jing, Man Li.Carbohydrate Content and Isozymes Changes during Fleshy Tap Root Formation and Thickening in Raphanus sativus. Acta Horticulturae,2007,763:275-282 (ISTP) (通讯作者).
Yin J, Guo W, Yang L, Liu L, Zhang T. Physical mapping of the Rf1 fertility-restoring gene to a 100 kb region in cotton. Theor Appl Genet, 2006,112: 1318–1325(SCI)
Wang T, Xu Steven S., Harris M O, Hu J, Liu L, Cai X.Genetic characterization and molecular mapping of Hessian fly resistance genes derived from Aegilops tauschii in synthetic wheat. Theor Appl Genet, 2006,113: 611-618(SCI)
Guang Liu, Liwang Liu*, Yiqin Gong, Yan Wang, Fanmin Yu, Haibin Shen,Weimin Gui. Seed Genetic Purity Testing of F1 Hybrid Cabbage (Brassica oleracea var.capitata) with Molecular Marker Analysis. Seed Sci & Tech., 2007,35:477-486(SCI). (通讯作者)
X. Li, L. Liu, Y. Gong, Y. Wang, B. Fu, X. Zhu, F. Yu and H. Shen. Molecular testing of cucumber hybrid genetic purity with RAPD marker. Seed science and Technology, Seed Sci. & Technol.,2008,36, 440-446(SCI)(通讯作者)
Jinlan Yang, Liwang Liu *, Yiqin Gong,et al. Cadmium uptake character and stress effect on the growth and antioxidant enzymes activities in Raphanus sativus. Acta Horticulturae 767, ISHS 2008:249-256 (ISTP) (通讯作者).
Liwang Liu*, Yan Wang , Yiqin Gong, Xin Zhai, Fanmin Yu, Haibin Shen. Genetic purity test of F1 hybrid tomato (Lycopersicon esculentum L.) using molecular marker analysis. Acta Horticulturae,2008,771: 231-238(ISTP) (通讯作者).
Ling-Li He, Li-Wang Liu*, Yi-Qin Gong, Xian-Wen Zhu, Jinlan Yang, Feng Wang, Bin Wu.Physiological Responses of Radish (Raphanus sativus L.) to Lead Stress.Bioinformatics and Biomedical Engineering(ICBBE 2008), 4602-4605, 2008 (EI)
Liwang Liu,Jinlan Yang,Xilin Hou*,Yiqin Gong,Xianwen Zhu,Feng Wang,Cuiping Zhang,Ruizhen Chu. Genotypic differences of Cd uptake and distribution in radish (Raphanus sativus L.). Bioinformatics and Biomedical Engineering (ICBBE 2008), 4696-4699, 2008 (EI)
F Wang, R Chu, J Yang, Y Gong*, X Zhu, Ch Zhu, L Xu, X He, L Liu*. Cadmium accumulation and antioxidant enzyme activity in response to cadmium stress in radish (Raphanus sativus L.). Bioinformatics and Biomedical Engineering (ICBBE 2009), 3rd International Conference on,11-13 June 2009 ,Page(s):1-4 .DOI 10.1109/ICBBE.2009.5162467 (EI) (通讯作者)
Xianyong Song, Jinlan Yang, Yiqin Gong,Liping Zhao,Danqiong Huang and Liwang Liu*. DNA Methylation Pattern in the Process of Floral Bud Differentiation in Radish (Raphanus sativus L.) with MSAP Analysis. Acta Horticulturae 774, ISHS 2008:409-416(ISTP) (通讯作者).
Liwang Liu*,Hao Huang,Yiqin Gong,Chongshun Chen, Xianyong Song.Cytological and ultra-structural study on microsporogenesis of cytoplasmic malesterility in Raphanus sativusL. Biologia.2009, 64(4):716-722 (SCI) (通讯作者)
Xinjuan Lu, Liwang. Liu*, Yiqin Gong, Liping Zhao, Xianyong Song, Xianwen Zhu.Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers. Scientia Horticulturae,2009 ,122: 645–648 (SCI)
R Chu, F Wang, Sh Li, Y Gong, X Zhu, L Xu, X Pu, L Liu*.Application of molecular makers in genetic diversity analysis of radish germplasm with different characters of Cd accumulation. Bioinformatics and Biomedical Engineering (ICBBE 2010), 4rd International Conference on,18-20 June 2010,Page(s): 1-4.Digital Object Identifier:10.1109/ICBBE.2010.5517385 (通讯作者), (EI)
ZHAO Li-Ping, LIU Li-Wang*,GONG Yi-Qin, WANG Ming-Xia, YU Fan-Min, WANG Long-zhi .Cultivar Fingerprinting in Radish (Raphanus sativus L.) with SRAP and AFLP Marker. BULLETIN OF BOTAN ICAL RESEARCH,2007,27(6):687-693 (通讯作者)
Zhao-liang Lu, Li-wang Liu* ,Xiao-yan Li, Yi-qin Gong, Xi-lin Hou, Xian-Wen Zhu , Long-zhi Wang. Analysis and evaluation of nutritional quality in Chinese radish(Raphanus sativus L.). Agricultural Sciences in China,2008,7(7):823-830
陈黎明,柳李旺*,晋萍,龚义勤,孙新菊,马二磊.两个萝卜雄性不育材料胞质的细胞学与分子鉴定分子植物育种,2009,7(4):757-762 (通讯作者)
邓代信,汪隆植,龚义勤,柳李旺*.萝卜雄性不育系几种同工酶研究. 西南农业学报, 2004,17(3):348-352(通讯作者)
龚义勤,李培,王明霞, 赵丽萍,柳李旺. 萝卜基因组DNA的RAMP-PCR体系优化.植物研究,2006,26(1):93-97 (通讯作者)
黄 浩,柳李旺*,陈崇顺,龚义勤,宋贤勇,韦开余.萝卜CMS不育系与保持系小孢子发生的细胞学研究. 植物研究,2004,24(3):305-308 (通讯作者)
黄浩,柳李旺*,陈崇顺,龚义勤,宋贤勇. 萝卜总RNA提取与mRNA差异显示技术.植物生理学通讯,2004,40(4):483-486(通讯作者)
荆赞革,柳李旺*, 龚义勤,姜立娜,孙新菊,周艳孔.萝卜扩展蛋白基因RsEXPB1 克隆与表达特征分析.分子植物育种,2009,7(4):801-805 (通讯作者)
柳李旺*,侯喜林,龚义勤. 分子标记技术在蔬菜作物品种鉴定与纯度检测中的应用.分子植物育种.2004,2(4):563-568
路昭亮,柳李旺,龚义勤,李小艳,杨金兰,汪隆植.萝卜干物重和可溶性总糖含量的遗传分析. 南京农业大学学报, 2009,32(3):25-29. (通讯作者)
宋贤勇,龚义勤,柳李旺,等.萝卜基因组DNA RAPD与ISSR-PCR反应体系优化. 种子,2007(2):1-5(通讯作者)
宋贤勇,柳李旺,龚义勤,黄丹琼,汪隆植.春萝卜抽薹过程中内源激素含量变化分析. 植物研究,2007,27(2):182-185 (通讯作者)
王玮,柳李旺*,龚义勤,等.萝卜肉质根膨大过程中碳水化合物与蔗糖代谢相关酶活性分析.园艺学报,2007,34(5):1313-1316 (通讯作者).
王燕, 龚义勤,柳李旺*.番茄SRAP-PCR体系优化与品种分子鉴定.南京农业大学学报,2007,30 (1) : 23~29(通讯作者)
杨金兰,柳李旺*,龚义勤,等.镉胁迫下萝卜基因组DNA甲基化敏感扩增多态性分析.植物生理与分子生物学学报,2007,33,(3):219-226 (通讯作者)
赵天荣, 龚义勤,柳李旺*.萝卜自交不亲和特性的荧光快速鉴定. 南京农业大学学报,2007,30(4):30-34(通讯作者)
著作教材
柳李旺(副主编).《中国萝卜》(国家科学技术学术著作出版基金资助出版).中国科学技术文献出版社,2005.7
柳李旺(参编).《园艺学各论》面向二十一世纪教材,中国农业出版社,2004
陈火英,柳李旺(主编). 《种子种苗学》,上海交通大学出版社,2011.7
柳李旺(参编).《园艺学各论》面向二十一世纪教材,中国农业出版社,2004
柳李旺(参编).《蔬菜育种与种子生产》,南京大学出版社,2000.5
发明专利
一种基于PCR技术的番茄杂交种纯度检测方法 ZL.200710020044.0 1/4
一种甘蓝种子遗传纯度的快速鉴定方法ZL.200710020045.5 1/4
一种获得萝卜单倍体的育种方法ZL. 200810123895.2 3/5
一种基于FLC基因的萝卜抽薹特性鉴定方法 申请号:200910032897.5 1/4
一种利用标记辅助选择进行萝卜CMS雄性不育系培育的方法 申请号: 201010266351.9,公开号CN 101956007A 1/6
一种糯玉米杂交种遗传纯度的快速鉴定方法 申请号:201110351885.6 1/6
品种鉴定
‘南春白5号’萝卜,通过江苏省农作物新品种鉴定(证书编号2010-2-3),江苏省农业委员会公告(第四十八次农作物品种审定会议鉴定公告)(第25号),2010.11.8
‘南春白6号’萝卜,通过江苏省农作物新品种鉴定(证书编号2010-2-4),江苏省农业委员会公告(第四十八次农作物品种审定会议鉴定公告)(第25号),2010.11.8
科研项目
国家科技支撑计划课题 萝卜雄性不育育种技术研究与新品种选育(2008BADB1B03). 2008.1-2010.12
国家自然科学基金 萝卜抽薹开花关键基因FLC/FT遗传特性与功能分析(31171956),2012.1-2014.12
国家自然科学基金 萝卜Cd吸收累积性状的遗传特性与相关基因表达分析(30972006),2009.9~2010.12
国家自然科学基金 萝卜晚抽薹性状遗传标记与相关基因的分离克隆(30300238),2004-2006
国家自然科学基金 萝卜肉质直根形成膨大的遗传学机理研究30571193 2006.1~2008.12
国家高技术研究发展计划(863计划)南方多湿地区蔬菜优质多抗分子聚合育种(萝卜专题)(2008AA10Z150), 2008-2010,
国家科技部农业科技成果转化资金项目 优质多抗春萝卜新品种示范与推广(2011GB23600006),2011.4-2013.4
国家科技支撑计划课题子课题,设施专用耐抽薹春萝卜高效育种体系建立及新品种选育2009BADB8B03-3,2009.1 -2011.12
蔬菜学(NCET-10-0473),教育部新世纪优秀人才支持计划资助, 2010.1-2012.12
江苏省科技支撑计划项目 优质多抗专用萝卜新品种选育(BE2010328),2010.10 -2013.6
江苏省高技术研究计划 优质抗病设施栽培萝卜新品种选育与育种技术研究BG2005314, 2005~2009
江苏省科技支撑计划项目 江苏主要蔬菜作物品种种子遗传纯度分子检测研究(BE2008312),2008-2011
江苏省农业种质资源基因库项目 江苏省十字花科蔬菜种质资源基因库建设(萝卜专题)[No. sx(2007)g13] 2007.7-2009.12
江苏省自然科学基金(创新人才) 萝卜晚抽薹性状分子遗传学机理研究BK2004418 2005.1~2007.12
教育部科学技术研究重点项目 萝卜镉吸收累积特性的遗传学特征鉴定(No.108068) ,2008.1-2010.12
上海农业“四新”重点项目(招标)出口西兰花、生菜安全生产技术开发[农字(2001-3-1)] ,2002-2005
上海市科技兴农重点攻关项目 主要蔬菜作物种子种苗质量的高效快速检测技术研究沪农科攻字(2004)第9-1号2004. 11~2009.11


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