兰州大学研究生导师介绍：郭光沁（Guang-Qin Guo）

　　学习经历

　　2000：兰州大学，博士；

　　1995-1996：德国马普研究所（科隆），欧盟玛丽.居里奖学金；

　　1987：兰州大学细胞生物学专业，硕士；

　　1984：兰州大学生物系，学士。

　　Ph.D. Lanzhou University, 2000.

　　Marie Curie scholarship, Max-Planck institute, Germany, 1995-1996.

　　M.S. Lanzhou University, 1987.

　　B.S. Lanzhou University, 1984.

　　工作经历

　　2003- ：兰州大学，教授，博士生导师；

　　1996-2003：兰州大学，副教授；

　　1990-1992：植物分子遗传国家重点实验室，客座，开放课题项目负责人；

　　1987-1996：山东大学，助教、讲师。

　　Professor, Lanzhou University, 2003-.

　　Associated Professor, Lanzhou University, 1996-2003.

　　Assistant Professor, Lecturer, Shandong University, 1987-1996.

　　Visiting scholar, State Key Laboratory of Plant Molecular Genetics, 1990-1992.

　　教学及指导研究生情况

　　现每年可同时招收硕士及博士研究生。

　　发表论文及专著

　　*correspondance

　　Di DW, Wu L, Zhang L, An CW, Zhang TZ, Luo P, Gao HH, Kriechbaumer V, Guo GQ*. 2016. Functional roles of Arabidopsis CKRC2/YUCCA8 gene and the involvement of PIF4 in the regulation of auxin biosynthesis by cytokinin. Sci Rep 6, 36866; doi: 10.1038/srep36866.

　　Di DW, Zhang C, Luo P, An CW, Guo GQ. 2015. The biosynthesis of auxin: How many paths truly lead to IAA? Plant Growth Regulation doi: 10.1007/s10725-015-0103-5.

　　Wu L, Pan L, Di DW, Wang L, Wang M, Lu CK, Wei SD, Zhang L, Zhang TZ, Amakorová P, Mrvková M, Novák O, Guo GQ*. 2015. Forward genetic screen for auxin-deficient mutants by cytokinin. Sci Rep 5, 11923; doi: 10.1038/srep11923.

　　Wu L, Zhou ZY, Zhang CG, Chai J, Zhou Q, Wang L, Hirnerová E, Mrvková M, Novák O, Guo GQ*. 2015. Functional roles of three cutin biosynthetic acyltransferases in cytokinin responses and skotomorphogenesis. PLoS One 10(3): e0121943; doi: 10.1371/journal.pone.0121943.

　　Di DW, Zhang C, Guo GQ. 2015. Involvement of secondary messengers and small organic molecules in auxin perception and signaling. Plant Cell Rep 34(6):895-904.

　　Wu L, Di D-W, Zhang D, Song B, Luo P, Guo GQ. 2015. Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes in Arabidopsis T-DNA tagged mutants. Biotechnol & Biotechnol Equipment 29(2): 260-267.

　　Zhao L, Liu P, Guo G*, Wang L*. 2015. Combination of cytokinin and auxin induces apoptosis, cell cycle progression arrest and blockage of the Akt pathway in HeLa cells. Mol Med Rep 12: 719-727.

　　Zhou ZY, Zhang CG, Wu L, Zhang CG, Chai J, Wang M, Jha A, Jia PF, Cui SJ, Yang M, Chen R, Guo GQ*. 2011. Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root. Plant J 66: 516–527.

　　Zhou Y, Zhang J, Lin H, Guo GQ, Guo Y. 2010. MORPHEUS’ MOLECULE1 is required to prevent aberrant RNA transcriptional read-through in Arabidopsis. Plant Physiol 154: 1272–1280.

　　Zhang CG, Guo HH, Zhang J, Guo GQ, Schumaker K, Guo Y. 2010. Arabidopsis CSAat1A and CSAat1B proteins form a complex with CULLIN4 and DDB1A and regulate the response to UV irradiation. Plant Cell 22: 2353-2369.

　　Liu Y, Yan Z, Chen N, Di X, Huang J, Guo G*. 2010. Development and function of central cell in angiosperm female gametophyte. Genesis (Dev Genet) 48: 466-478.

　　Wang QF, Li W, Yang Hai, Liu YL, Cao HH, Dornmayr-Pfaffenhuemer M, Stan-Lotter H, Guo GQ*, 2007, Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample. Int J Syst Evol Microbiol 57: 600-604.

　　Tiwari RK*, Trivedi M, Guang ZC, Guo GQ*, Zheng GC. 2007. Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures. Plant Cell Rep 26: 199–210.

　　Guo GQ*, Zheng GC, 2004, Hypotheses for the functions of intercellular bridges in male germ cell development and its cellular mechanisms. J Theor Biol 229: 139-146.

　　Guo GQ, Maiwald F, Lorenzen P & Steinbiss HH, 1998, Factors influencing T-DNA transfer into wheat and barley cells by Agrobacterium tumefaciens. Cereal Res Comm 26: 15-22.

　　郭光沁、许智宏、卫志明、陈惠民，1993，用PEG法向小麦原生质体导入外源基因获得转基因植株。科学通报 38(13): 1227-31。

　　Guo GQ, Xia GM, Li ZY, Chen HM, 1991, Direct somatic embryogenesis and plant regeneration from protoplast-derived cells of wheat. Sci in China (ser B) 34: 438-45.

　　研究方向

　　Research Interests

　　1、 细胞分裂素和生长素相互作用 Auxin/cytokinin interactions

　　生长素和细胞分裂素是调控植物发育的两类重要激素，其比例和相互作用决定着细胞生长和分化的命运。我们用分子遗传学等相关技术研究细胞分裂素如何影响生长素生物合成及极性运输进而调控植物的生长发育。

　　Auxin and cytokinin are the two classic plant hormones playing pivotal roles in plant development. It is a famous theme in plant science that the development of cells into roots or shoots is often determined by the ratio of auxin/cytokinin. We use molecular genetics to studies how cytokinin regulates auxin biosynthesis and its polar transport to influence plant development.

　　2. 生殖细胞发育 Germ cell development

　　植物的有性生殖涉及减数分裂（孢子发生）和配子体（花粉）发育，受多个基因调控并需要细胞间的通讯和相互协调。我们主要用拟南芥为材料分离突变体，用分子生物学手段研究大小孢子发生及其配子体发育的分子机理。

　　Sexual reproduction in plants occurs via meiosis (sporogenesis) and gametophyte development under strict genetic control with highly coordination between cells. We use the current powerful tools in cell and molecular biology to study the related molecular mechanisms through isolating mutants in Arabidopsis and cloning the corresponding genes.

　　项目成果

　　先后主持国家自然科学基金面上、重大研究计划和重点项目，以及973项目子课题。目前主持2项国家自然基金在研项目：

　　（1）酰基转移酶GFC1参与细胞分裂素信号转导的生化和分子机理；

　　（2）CKRC3在维持拟南芥内源生长素稳态中的作用和机理。

　　生长素和细胞分裂素是调控植物生长发育的重要激素，对它们作用机理的研究一直是国际上的热点。

　　研究成果：（1）是国际上独立发现生长素生物合成IPA途径突变体taa1的4家实验室之一，并进一步独立提出了细胞分裂素通过促进生长素合成并抑制其极性运输的作用机理调控植物发育[Plant J, 66, 516–527 (2011)]；(2）2004年提出胞间桥/通道功能假说（Guo & Zheng 2004），在国际上受到关注，被《Cell》、《PNAS》、《Trends Mol Med》、《Biol Rev》、《Cold Spring Harb Perspect Biol》和《Dev Biol》及相关专著所引用；（3）1990年在国际上公认为难度极大的小麦原生质体培养中首先实现再生细胞直接形成体细胞胚和再生植株。

　　荣誉、获奖

　　社会工作

　　2013：细胞研究所所长

　　2012：《西北植物学报》编委